ABSTRACT
OBJECTIVE: To prepare Panax notoginseng saponins-loaded complex nano vesicles (PNS-CNV) with MPEG-PLGA nanoparticles as the core and investigate the physicochemical property of PNS-CNV and protective action on acute myocardial ischemia in rats. METHODS: Double emulsification-solvent evaporation method was used to prepare Panax notoginseng saponins-loaded nanoparticles using different molecular weight MPEG-PLGA (PNS-NP). Thin-film hydration method was applied to entrap PNS-NP as the core to form PNS-CNV. Ultrafiltration, transmission electron microscope, dynamic light scattering and electrophoretic light scattering were employed to study the entrapment efficiency of each index component in PNS and physicochemical parameters of PNS-CNV. Acute myocardial ischemia model in rats was established to investigate the protective effect of PNS-CNV. RESULTS: The molecular weight of MPEG-PLGA did not affect the entrapment efficiency, particle size and Zeta potential of PNS-CNV. The pharmacodynamic experiment indicated that comparing with PNS solution group, the SOD activity of rat myocardium in PNS-CNV group was elevated with markedly decreased H2O2, MDA and serum LDH activity (P<0.05). The protective effect of PNS-CNV with MPEG5000-PLGA was the most significant. CONCLUSION: CNV may be used as potential new oral drug delivery carriers.